Lessons learned from counting molecules: how to lure CENP-A into the kinetochore
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چکیده
Commentary Cite this article: Scott KC, Bloom KS. 2014 Lessons learned from counting molecules: how to lure CENP-A into the kinetochore. Open Biol.
منابع مشابه
CENP-A exceeds microtubule attachment sites in centromere clusters of both budding and fission yeast
The stoichiometries of kinetochores and their constituent proteins in yeast and vertebrate cells were determined using the histone H3 variant CENP-A, known as Cse4 in budding yeast, as a counting standard. One Cse4-containing nucleosome exists in the centromere (CEN) of each chromosome, so it has been assumed that each anaphase CEN/kinetochore cluster contains 32 Cse4 molecules. We report that ...
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CENP-A acts as an important epigenetic marker for kinetochore specification. However, the mechanisms by which CENP-A is incorporated into centromeres and the structural basis for kinetochore formation downstream of CENP-A remain unclear. Here, we used a unique chromosome-engineering system in which kinetochore proteins are targeted to a noncentromeric site after the endogenous centromere is con...
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Kinetochore specification and assembly requires the targeted deposition of specialized nucleosomes containing the histone H3 variant CENP-A at centromeres. However, CENP-A is not sufficient to drive full-kinetochore assembly, and it is not clear how centromeric chromatin is established. Here, we identify CENP-W as a component of the DNA-proximal constitutive centromere-associated network (CCAN)...
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عنوان ژورنال:
دوره 4 شماره
صفحات -
تاریخ انتشار 2014